Which condition leads to a reduced affinity of hemoglobin for oxygen, known as the Bohr effect?

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1. Which condition leads to a reduced affinity of hemoglobin for oxygen, known as the Bohr effect?

1. Increased pH2. Low temperature3. High CO₂ and low pH4. Low CO₂ and high pH

Which condition leads to a reduced affinity of hemoglobin for oxygen, known as the Bohr effect?